p16 and Ki-67 are two biomarkers that indicate the presence of transforming HPV infections. The use of p16/Ki-67 dual staining in the triage of HPV positive results leads to better disease detection, allocation of limited healthcare resources, and peace of mind for the patient. Simultaneously detecting p16 and Ki-67 can also help clinicians recommend follow-up for women who will benefit most from colposcopy, resulting in more disease detection without increasing the colposcopy rate.